| Topic | Concepts | Experiment |
| Spectrophotometry |
The
basic principles of spectrophotometry and the operation of a
spectrophotometer are explained. | The
transmittance and absorbance are measured for a sample. A blank is used
to calibrate the spectrophotometer. |
| Absorbance
Spectrum | The
absorbance spectrum is described and its importance explained. |
The
absorbance spectrum for a sample is measured. |
| Effect
of Cell Path Length | The
significance of the cell path length is discussed. | The
effect of the cell path length on transmittance and absorbances is
explored. |
| Effect
of Concentration | The
significance of the analyte concentration is discussed. | The
effect of the analyte concentration on transmittance and absorbances is
explored. |
| Beer's
Law | Beer's
Law is presented and explained. | A
plot of absorbance vs concentration is prepared, and the molar
absorptivity is determined from the slope of the plot. |
| Analysis of an
Unknown Solution | The
construction of a
calibration curve is described, and the use of a calibration curve in
determining the analyte concentration in an unknown solution is
explained. | A
calibration curve is prepared and used to determine the analyte
concentration in an unknown solution. |